SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-05-22 13:51:54

locus

BSU07840

BSU_07840

geneLength

4386

4389

outlinks

bsu

BSU07840

BSU_07840

The protein

Catalyzed reaction/ biological activity

degrades c-di-AMP two 5′ AMP molecules [Pubmed|27414497]

degrades c-di-AMP two 5′ AMP molecules [Pubmed|27414497]

nucleoside 2',3'-cyclic phosphate + H2O --> nucleoside 3'-phosphate + H+ (according to UniProt)

ribonucleoside 3'-phosphate + H2O --> ribonucleoside + phosphate (according to UniProt)

ribonucleoside 5'-phosphate + H2O --> ribonucleoside + phosphate (according to UniProt)

The protein

Paralogous protein(s)

[[protein|YhcR]]

[[this]]

The protein

Structure

[PDB|3GVE]

[PDB|2Z1A] (from Thermus thermophilus, 40% identity)

[PDB|3GVE] (fragment, aa 37 - 374)

Biological materials

Mutant

GP2026 (Δ''yfkN::aphA3''), available in [SW|Jörg Stülke]'s lab [Pubmed|27681641]

MGNA-C263 (yfkN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2261 NBRP B. subtilis, Japan]

GP2026 (Δ''yfkN::aphA3''), available in [SW|Jörg Stülke]'s lab [Pubmed|27681641]

MGNA-C263 (yfkN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2261 NBRP B. subtilis, Japan]

BKE07840 (Δ[[gene|yfkN]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCAGTTCTCCACCTTTCA, downstream forward: _UP4_TAAAAAAGAGCTGCTCGCTA

BKK07840 (Δ[[gene|yfkN]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCAGTTCTCCACCTTTCA, downstream forward: _UP4_TAAAAAAGAGCTGCTCGCTA

The protein

Protein family

5'-nucleotidase family (with [[protein|YhcR]], according to UniProt)